Fronzes Lab

Publications

Our recent publications:

 

Cherrak Y, Rapisarda C, Pellarin R, Bouvier G, Bardiaux B, Allain F, Malosse C, Rey M, Chamot-Rooke J, Cascales E, Fronzes R, Durand E. 2018.
Biogenesis and structure of a type VI secretion baseplate.
Nat Microbiol 2018

 

Rapisarda C, Cherrak Y, Kooger R, Schmidt V, Pellarin R, Logger L, Cascales E, Pilhofer M, Durand E, Fronzes R. 2019.
In situ and highresolution cryo EM structure of a bacterial type VI secretion system membrane complex .
EMBO J 2019 

 

Pony P, Rapisarda C, Terradot L, Marza E, Fronzes R. 2020.
Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation.
Nat. Commun, 2020 

 

Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E, Fronzes R, Journet L. 2020
Structural Basis for Loading and Inhibition of a Bacterial T6SS Phospholipase Effector by the VgrG Spike
EMBO J. 2020 

2020

Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E, Fronzes R, Journet L.
Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike.
EMBO J. 2020 Jun 2;39(11):e104129. doi: 10.15252/embj.2019104129. Epub 2020 Apr 30. PMID: 32350888; PMCID: PMC7265238.

 

Pony P, Rapisarda C, Terradot L, Marza E, Fronzes R.
Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation.
Nat Commun. 2020 Mar 18;11(1):1426. doi: 10.1038/s41467-020-15214-y. PMID: 32188856; PMCID: PMC7080775.

 

Kumar A, Planchais C, Fronzes R, Mouquet H, Reyes N.
Binding mechanisms of therapeutic antibodies to human CD20.
Science. 2020 Aug 14;369(6505):793-799.
doi: 10.1126/science.abb8008. PMID: 32792392.

2019

Rapisarda C, Cherrak Y, Kooger R, Schmidt V, Pellarin R, Logger L, Cascales E, Pilhofer M, Durand E, Fronzes R.
In situ and high-resolution cryo-EM structure of a bacterial type VI secretion system membrane complex.
EMBO J. 2019 May 15;38(10):e100886. doi: 10.15252/embj.2018100886. Epub 2019 Mar 15.

 

Perry TN, Souabni H, Rapisarda C, Fronzes R, Giusti F, Popot JL, Zoonens M, Gubellini F.
BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy.
Biochim Biophys Acta Biomembr. 2019 Feb 1;1861(2):466-477. doi:10.1016/j.bbamem.2018.11.004.

2018

Cherrak Y, Rapisarda C, Pellarin R, Bouvier G, Bardiaux B, Allain F, Malosse C, Rey M, Chamot-Rooke J, Cascales E, Fronzes R, Durand E.
Biogenesis and structure of a type VI secretion baseplate.
Nat Microbiol. 2018 Dec;3(12):1404-1416. doi: 10.1038/s41564-018-0260-1. Epub 2018 Oct 15. PMID: 30323254.

 

Rapisarda C, Tassinari M, Gubellini F, Fronzes R.
Using Cryo-EM to Investigate Bacterial Secretion Systems.
Annu Rev Microbiol. 2018 Sep 8;72:231-254. doi: 10.1146/annurev-micro-090817-062702. Epub 2018 Jul 13. PMID: 30004822.

2017

Krasteva PV, Bernal-Bayard J, Travier L, Martin FA, Kaminski PA, Karimova G, Fronzes R, Ghigo JM.
Insights into the structure and assembly of a bacterial cellulose secretion system.
Nat Commun. 2017 Dec 12;8(1):2065. doi: 10.1038/s41467-017-01523-2. PubMed PMID: 29234007; PubMed Central PMCID: PMC5727187

 

Koelblen T, Bergé C, Cherrier MV, Brillet K, Jimenez-Soto L, Ballut L, Takagi J, Montserret R, Rousselle P, Fischer W, Haas R, Fronzes R, Terradot L.
Molecular dissection of protein-protein interactions between integrin α5β1 and the Helicobacter pylori Cag type IV secretion system.
FEBS J. 2017 Dec;284(23):4143-4157. doi: 10.1111/febs.14299. Epub 2017 Nov 12. PubMed PMID: 29055076.

 

Diallo A, Foster HR, Gromek KA, Perry TN, Dujeancourt A, Krasteva PV, Gubellini F, Falbel TG, Burton BM, Fronzes R.
Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA.
Mol Microbiol. 2017 Sep;105(5):741-754. doi: 10.1111/mmi.13732. Epub 2017 Jul 28. PubMed PMID: 28618091.

Marie L, Rapisarda C, Morales V, Bergé M, Perry T, Soulet AL, Gruget C, Remaut H, Fronzes R, Polard P.
Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension.
Nat Commun. 2017 May 31;8:15638. doi: 10.1038/ncomms15638. PubMed PMID: 28561029; PubMed Central PMCID: PMC5512693.

 

Gubellini F, Fronzes R.
Labeling of Membrane Complexes for Electron Microscopy.
Methods Mol Biol. 2017;1635:125-138. doi: 10.1007/978-1-4939-7151-0_7. PubMed PMID: 28755367.

2016

Wojtowicz H, Prochnicka-Chalufour A, de Amorim GC, Roudenko O, Simenel C, Malki I, Pehau-Arnaudet G, Gubellini F, Koutsioubas A, Pérez J, Delepelaire P, Delepierre M, Fronzes R, Izadi-Pruneyre N.
Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach.
Biochem J. 2016 Jul 15;473(14):2239-48. doi: 10.1042/BCJ20160131. Epub 2016 May 13. PubMed PMID: 27208170; PubMed Central PMCID: PMC4941744.

 

Zoued A, Durand E, Brunet YR, Spinelli S, Douzi B, Guzzo M, Flaugnatti N, Legrand P, Journet L, Fronzes R, Mignot T, Cambillau C, Cascales E.
Priming and polymerization of a bacterial contractile tail structure.
Nature. 2016 Mar 3;531(7592):59-63. doi: 10.1038/nature17182. Epub 2016 Feb 24. PubMed PMID: 26909579.

2015

Durand E, Nguyen VS, Zoued A, Logger L, Péhau-Arnaudet G, Aschtgen MS, Spinelli S, Desmyter A, Bardiaux B, Dujeancourt A, Roussel A, Cambillau C, Cascales E, Fronzes R.
Biogenesis and structure of a type VI secretion membrane core complex.
Nature. 2015 Jul 30;523(7562):555-60. doi: 10.1038/nature14667. Epub 2015 Jul 22. PubMed PMID: 26200339.

 

Kaplan M, Cukkemane A, van Zundert GC, Narasimhan S, Daniëls M, Mance D, Waksman G, Bonvin AM, Fronzes R, Folkers GE, Baldus M.
Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Nat Methods. 2015 Jul;12(7):649-52. doi: 10.1038/nmeth.3406. Epub 2015 May 18. PubMed PMID: 25984698.

 

Laurenceau R, Krasteva PV, Diallo A, Ouarti S, Duchateau M, Malosse C, Chamot-Rooke J, Fronzes R.
Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence.
PLoS Pathog. 2015 Apr 15;11(4):e1004835. doi: 10.1371/journal.ppat.1004835. eCollection 2015 Apr. PubMed PMID: 25876066; PubMed Central PMCID: PMC4398557.

2014

Goyal P, Krasteva PV, Van Gerven N, Gubellini F, Van den Broeck I, Troupiotis-Tsaïlaki A, Jonckheere W, Péhau-Arnaudet G, Pinkner JS, Chapman MR, Hultgren SJ, Howorka S, Fronzes R, Remaut H.
Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG.
Nature. 2014 Dec 11;516(7530):250-3. doi: 10.1038/nature13768. Epub 2014 Sep 14. PubMed PMID: 25219853; PubMed Central PMCID: PMC4268158.

 

Low HH, Gubellini F, Rivera-Calzada A, Braun N, Connery S, Dujeancourt A, Lu F, Redzej A, Fronzes R, Orlova EV, Waksman G.
Structure of a type IV secretion system.
Nature. 2014 Apr 24;508(7497):550-553. doi: 10.1038/nature13081. Epub 2014 Mar 9. PubMed PMID: 24670658; PubMed Central PMCID: PMC3998870.

2013

Laurenceau R, Péhau-Arnaudet G, Baconnais S, Gault J, Malosse C, Dujeancourt A, Campo N, Chamot-Rooke J, Le Cam E, Claverys JP, Fronzes R.
A type IV pilus mediates DNA binding during natural transformation in Streptococcus pneumoniae.
PLoS Pathog. 2013;9(6):e1003473. doi: 10.1371/journal.ppat.1003473. Epub 2013 Jun 27. PubMed PMID: 23825953; PubMed Central PMCID: PMC3694846.

 

Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV.
Structure of a bacterial type IV secretion core complex at subnanometre resolution.
EMBO J. 2013 Apr 17;32(8):1195-204. doi: 10.1038/emboj.2013.58. Epub 2013 Mar 19. PubMed PMID: 23511972;PubMed Central PMCID: PMC3630358.

 

Daviter T, Fronzes R.
Protein sample characterization.
Methods Mol Biol. 2013;1008:35-62. doi: 10.1007/978-1-62703-398-5_2. PubMed PMID: 23729248

2012

Baranova E, Fronzes R, Garcia-Pino A, Van Gerven N, Papapostolou D, Péhau-Arnaudet G, Pardon E, Steyaert J, Howorka S, Remaut H.
SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly.
Nature. 2012 Jul 5;487(7405):119-22. doi: 10.1038/nature11155. PubMed PMID: 22722836.

2010

Waksman G, Fronzes R.
Molecular architecture of bacterial type IV secretion systems.
Trends Biochem Sci. 2010 Dec;35(12):691-8. doi: 10.1016/j.tibs.2010.06.002. Epub 2010 Jul 10. Review. PubMed PMID: 20621482.

 

Rego AT, Fronzes R, Waksman G.
SnapShot: Bacterial appendages II.
Cell. 2010 Jan 22;140(2):294-294.e1. doi: 10.1016/j.cell.2010.01.006. PubMed PMID: 20141842.

 

Rego AT, Fronzes R, Waksman G.
SnapShot: Bacterial Appendages I.
Cell. 2010 Jan 8;140(1):162-162.e1. doi: 10.1016/j.cell.2009.12.047. PubMed PMID: 20085708.

2009

Chandran V, Fronzes R, Duquerroy S, Cronin N, Navaza J, Waksman G.
Structure of the outer membrane complex of a type IV secretion system.
Nature. 2009 Dec 24;462(7276):1011-5. doi: 10.1038/nature08588. Epub 2009 Nov 29. PubMed PMID: 19946264; PubMed Central PMCID: PMC2797999.

 

Fronzes R, Christie PJ, Waksman G.
The structural biology of type IV secretion systems.
Nat Rev Microbiol. 2009 Oct;7(10):703-14. doi: 10.1038/nrmicro2218. Review. PubMed PMID: 19756009; PubMed Central PMCID: PMC3869563.

 

Meng G, Fronzes R, Chandran V, Remaut H, Waksman G.
Protein oligomerization in the bacterial outer membrane (Review).
Mol Membr Biol. 2009 Apr;26(3):136-45. doi: 10.1080/09687680802712422. Epub 2009 Feb 14. Review. PubMed PMID: 19225986.

 

Fronzes R, Schäfer E, Wang L, Saibil HR, Orlova EV, Waksman G.
Structure of a type IV secretion system core complex.
Science. 2009 Jan 9;323(5911):266-8. doi: 10.1126/science.1166101. PubMed PMID: 19131631.

2008

Fronzes R, Remaut H, Waksman G.
Architectures and biogenesis of non-flagellar protein appendages in Gram-negative bacteria.
EMBO J. 2008 Sep 3;27(17):2271-80. doi: 10.1038/emboj.2008.155. Review. PubMed PMID: 18668121; PubMed Central PMCID: PMC2500206.

 

Backert S, Fronzes R, Waksman G.
VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems?
Trends Microbiol. 2008 Sep;16(9):409-13. doi: 10.1016/j.tim.2008.07.001. Epub 2008 Aug 14. PubMed PMID: 18706815.

 

Goyon V, Fronzes R, Salin B, di-Rago JP, Velours J, Brèthes D.
Yeast cells depleted in Atp14p fail to assemble Atp6p within the ATP synthase and exhibit altered mitochondrial cristae morphology.
J Biol Chem. 2008 Apr 11;283(15):9749-58. doi: 10.1074/jbc.M800204200. Epub 2008 Feb 5. PubMed PMID: 18252710; PubMed Central PMCID: PMC2442300.

2007

Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G.
NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system.
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PubMed PMID: 17244707; PubMed Central PMCID: PMC1785264.

2006

Fronzes R, Weimann T, Vaillier J, Velours J, Brèthes D.
The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits.
Biochemistry. 2006 May 30;45(21):6715-23. PubMed PMID: 16716082.

2003

Fronzes R, Chaignepain S, Bathany K, Giraud MF, Arselin G, Schmitter JM, Dautant A, Velours J, Brèthes D.
Topological and functional study of subunit h of the F1Fo ATP synthase complex in yeast Saccharomyces cerevisiae.
Biochemistry. 2003 Oct 21;42(41):12038-49. PubMed PMID: 14556635.