Fronzes Lab

Structure & function

of bacterial nanomachines

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Welcome to the Fronzes Lab

The Team Structure and function of bacterial nanomachines is part of the UMR 5234 Microbiologie fondamentale et pathogénicité and is located in the Institut Européen de Chimie et Biologie in Pessac, France

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Our research themes

Bacterial type VI

secretion systems

Metabolic adaptability of bacterial pathogens

Bacterial transformation

systems

Other bacterial nanomachines

Latest News

Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike

 

The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. The mode of action of the T6SS is similar to contractile phages: the contraction of a sheath structure pushes a tube topped by a spike into target cells. Effectors are loaded onto the spike or confined into the tube. In enteroaggregative Escherichia coli , the Tle1 phospholipase binds the C‐terminal extension of the VgrG trimeric spike. Here, we purify the VgrG–Tle1 complex and show that a VgrG trimer binds three Tle1 monomers and inhibits their activity. Using covalent cross‐linking coupled to high‐resolution mass spectrometry, we provide information on the sites of contact and further identify the requirement for a Tle1 N‐terminal secretion sequence in complex formation. Finally, we report the 2.6‐Å‐resolution cryo‐electron microscopy tri‐dimensional structure of the (VgrG)3–(Tle1)3 complex revealing how the effector binds its cargo, and how VgrG inhibits Tle1 phospholipase activity. The inhibition of Tle1 phospholipase activity once bound to VgrG suggests that Tle1 dissociation from VgrG is required upon delivery.

 

Published in EMBO journal

 

Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E, Fronzes R, Journet L. 2020
Structural Basis for Loading and Inhibition of a Bacterial T6SS Phospholipase Effector by the VgrG Spike
EMBO J. 2020

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To #CryoEM tweeps. We are considering to buy a new camera attached to an post-column energy filter for a 200 kv microscope (Talos or Glacios). We are looking for feedback for Gatan vs TFS systems.

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