Fronzes Lab

Structure & function

of bacterial nanomachines

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Welcome to the Fronzes Lab

The Team Structure and function of bacterial nanomachines is part of the UMR 5234 Microbiologie fondamentale et pathogénicité and is located in the Institut Européen de Chimie et Biologie in Pessac, France

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Our research themes

Bacterial type VI

secretion systems

Metabolic adaptability of bacterial pathogens

Bacterial transformation

systems

Other bacterial nanomachines

Latest News

The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

 

 

Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their VH domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a « hug of death » mechanism. As a result, the orientation of the VL and VH domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.

 

Published in Science Advances

 

Nottelet P, Bataille L, Gourgues G, Anger R, Lartigue C, Sirand-Pugnet P, Marza E, Fronzes R, Arfi Y.


The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction.


Sci Adv. 2021 Mar 5;7(10)

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.@QuintanaMurci : "Pour mes travaux (sur l'immunité des populations), je reçois de l'argent de @institutpasteur, du @cdf1530... Mais de l'Etat, non. La raison invoquée par l'ANR : "Projet pas intéressant, manque d'hypothèses claires." Nature vient de me publier."

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Towards Visual Proteomics at High Resolution - ScienceDirect https://www.sciencedirect.com/science/article/pii/S0022283621004204

Online now! Biosensors responding to protein conformation enable single molecule detection of active proteins in live cells
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